An improved light-sheet microscope footage dwell cells at sub-100-nm axial resolution.
Contemporary light microscopy ways designed to make stronger spatial resolution are in general less widely relevant than anticipated on account of elevated phototoxicity in living samples. Writing in Nature Biotechnology, Cao et al.1 file a expertise that a great deal increases the resolution of light-sheet microscopy with out sacrificing its low phototoxicity and excessive sensitivity. The style, known as three-d interferometric lattice light-sheet (3D-iLLS) imaging, improves axial resolution thru the coherent dispute of two detection objectives and structured illumination with optical lattices. The authors demonstrate dwell cell imaging with sub-100-nm axial resolution in a gradual-weight-sheet structure and single-molecule localization microscopy with sub-10-nm axial precision.
Receive entry to suggestions
Subscribe to Journal
Receive elephantine journal safe admission to for 1 year
99,00 €
most productive 8,25 € per peril
Tax calculation will be finalised at some level of checkout.
Rent or Take article
Receive time restricted or elephantine article safe admission to on ReadCube.
from$8.99
All costs are NET costs.
References
- 1.
Cao, B., Wang, G., Li, J. & Pertsinidis, A. Nat. Biotechnol. https://doi.org/10.1038/s41587-021-01042-y (2021).
- 2.
Hell, S. W., Stelzer, E. H. Okay., Lindek, S. & Cremer, C. Opt. Lett. 19, 222–224 (1994).
- 3.
Gustafsson, M. G., Agard, D. A. & Sedat, J. W. J. Microsc. 195, 10–16 (1999).
- 4.
Shtengel, G. et al. Proc. Natl Acad. Sci. USA 106, 3125–3130 (2009).
- 5.
Chen, B.-C. et al. Science 346, 1257998 (2014).
- 6.
Chang, B.-J., Dean, Okay. M. & Fiolka, R. Opt. Categorical 28, 27052–27077 (2020).
- 7.
Hell, S. W., Lindek, S. & Stelzer, E. H. Okay. J. Mod. Opt. 41, 675–681 (1994).
- 8.
Shao, L. et al. Biophys. J. 94, 4971–4983 (2008).
Acknowledgements
The author is grateful for funding by the National Institutes of Neatly being (R35GM133522) and thanks James Manton for relief simulating the 4Pi level spread feature old-fashioned in Fig. 1b,d.
Ethics declarations
Competing interests
The author declares no competing interests.
About this text
